.:: Biotechnology and Food Science

Article details

Journal

Zeszyty naukowe PŁ, Chemia Spożywcza i Biotechnologia
Zeszyt 72

Year

2008

Title

The study of interactions between diclofenac and bovine serum albumin (BSA)

Authors

Joanna Jabłońska^a
Daniela Casares^b
Johanna Fünfer^c
Thomas Habenschuss^d
Gabriela Jimenez^b
Anna-Lena Klemm^e
Antônio Magela Machado Junior^f
Sandra Miloshevska^g
Ivana Papišta^h
Sofie Louise Ulrich-Lyngeⁱ
^aInstitute of General Food Chemistry, Technical University of Lodz, Poland
^bSan Francisco of Quito University, Quito, Ecuador
^cMartin-Luther-Universität Halle-Wittenberg, Germany
^dUniversity of Vienna, Vienna, Austria
^eRheinische Friedrich Willhelms-Universität Bonn, Bonn, Germany
^fUniversidade Federal de Ouro Preto, Brasil
^gSs. Cyril and Methodius University Skopje, Skopje, Macedonia
^hUniversity of Zagreb, Zagreb, Croatia
ⁱTechnical University of Denmark, Denmark

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Pages: 45 - 61

Abstract

Investigation of the interactions between diclofenac and BSA was performed using UV-Vis absorption and fluorescence methods. There was a quenching effect found between these two molecules in the fluorescence measurement methods; steady-state and time-resolved. The fluorescence decay time enables to determine the quenching mechanism and thus conclude that the complex is formed in groundstate but also collisional quenching -when the excited-state fluorophore of BSA is deactivated- occurs. Computer modeling was applied to observe and optimize the formation of the complex between N-acetyl-Ltryptophanamide and diclofenac.

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