Article details

Journal
Biotechnology and Food Science
Volume 80 Number 1
Year
2016
Title
Detection of meat adulteration in veal sausages using a multiplex PCR technique
Authors

Wojciech Sawicki,1* Joanna Żochowska-Kujawska2
1 Department of Microbiology and Applied Biotechnology, West Pomeranian University of Technology in Szczecin, Papieża Pawła VI 3, 71-459, Poland
2Department of Meat Science, West Pomeranian University of Technology in Szczecin, Kazimierza Królewicza 4, 71-550 Szczecin, Poland
* wojciech.sawicki@zut.edu.pl

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Pages: 19-27
Abstract

Previous methods used to detect veal contamination by lower value meat lacked easiness and accuracy This study describes a simple procedure for detecting origin of meat in processed meat products. A rapid (5h) protocol based on multiplex PCR was developed to detect undeclared chicken meat (a specific mitochondrial DNA sequence) in Polish veal sausages. Simultaneously a PCR assay was successfully optimized for amplification of 274-bp DNA fragment extracted from meat products using designed species-specific primer pairs for the detection of veal meat. This procedure also enabled differentiation of bovine or/and chicken meat from their less expensive porcine substitute.

Keywords
food adulterations, meat, multiplex PCR
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